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| Wu,Dan; Zhang,Jinli; Fan,Peiwen; Li,Hongtao; Li,Dongmei; Pan,Huan; He,Hongchang; Ren,Xianxian; Pan,Zhenzhen; Shao,Renfu; Pan,Zemin. |
| Abstract This study aimed to explore: 1) DNA methylation in the promoter regions of Wilms tumor gene 1 (WT1), NK6 transcription factor related locus 1 gene (NKX6-1) and Deleted in bladder cancer 1 (DBC1) gene in cervical cancer tissues of Uygur women in Xinjiang, and 2) the correlation of gene methylation with the infection of HPV16/18 viruses. We detected HPV16/18 infection in 43 normal cervical tissues, 30 cervical intraepithelial neoplasia lesions (CIN) and 48 cervical cancer tissues with polymerase chain reaction (PCR) method. Methylation in the promoter regions of the WT1, NKX6-1 and DBC1 genes in the above-mentioned tissues was measured by methylation-specific PCR (MSP) and cloning sequencing. The expression level of these three genes was measured by... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Gene methylation; Gene expression; HPV16/18; Cervical cancer; Uygur women. |
| Ano: 2018 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572018000100009 |
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| Zhang,Xian; Wu,Dan; Yang,Taowei; Xu,Meijuan; Rao,Zhiming. |
| Background: 3-Ketosteroid-∆¹-dehydrogenase (KSDD), a flavoprotein enzyme, catalyzes the bioconversion of 4-androstene-3,17-dione (AD) to androst-1,4-diene-3,17-dione (ADD). To date, there has been no report about characterization of KSDD from Mycobacterium neoaurum strains, which were usually employed to produce AD or ADD by fermentation. Results: In this work, Corynebacterium crenatum was chosen asa new host for heterologous expression of KSDD from M. neoaurum JC-12 after codon optimization of the KSDD gene. SDS-PAGE and western blotting results indicated that the recombinant C. crenatum harboring the optimized ksdd (ksdd n) gene showed significantly improved ability to express KSDD. The expression level of KSDD was about 1.6-fold increased C.... |
| Tipo: Journal article |
Palavras-chave: Androst-1; 4-diene-3; 17-dione Bioconversion Codon optimization Flavoprotein enzyme Heterologous expression Mycobacterium neoaurum Overexpression Recombinant Corynebacterium Sterol catabolism Whole-cell catalyst. |
| Ano: 2016 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582016000600013 |
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